Rheumatoid Arthritis: Methods and Protocols: 1868 (Methods in Molecular Biology)

 

Description:

 

This volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment.

Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study.

 

Table of contents:

 

Collagen-Induced Arthritis Models

Pages 3-7

Miyoshi, Maya (et al.

)

 

Human Xenograft Model

Pages 9-15

Liu, Shuang

 

Long-Term Constant Subcutaneous Drug Administration

Pages 17-22

Liu, Shuang (et al.

)

 

Clinical Scoring of Disease Activity in Animal Models

Pages 23-28

Miyoshi, Maya (et al.

)

 

Histological Analysis of Arthritic Joints

Pages 29-39

Kiyoi, Takeshi

 

Preparation of Joint Extracts

Pages 41-45

Liu, Shuang (et al.

)

 

Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System

Pages 49-67

Zhou, Wei (et al.

)

 

Reconstruction of Protein/Liposome Complex

Pages 69-77

Suzuki, Yasuyuki

 

Production of Neutralizing Antibody

Pages 79-92

Takemasa, Erika (et al.

)

 

Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen

Pages 93-112

Takeda, Hiroyuki

 

Generation of Specific Aptamers

Pages 113-121

Liu, Shuang (et al.

)

 

Production of Lentiviral Particles

Pages 123-128

Liu, Shuang

 

RNA Interference Ex Vivo

Pages 129-135

Liu, Shuang

 

Lentiviral-Mediated Systemic RNA Interference In Vivo

Pages 137-144

Liu, Shuang

 

Mesenchymal Stem Cell Engineering

Pages 145-150

Liu, Shuang

 

Screening of Ca2+ Influx in Lymphocytes

Pages 153-159

Takemasa, Erika (et al.

)

 

Single-Cell Ca2+ Imaging

Pages 161-168

Liu, Shuang

 

Electrophysiological Methods to Measure Ca2+ Current

Pages 169-176

Liu, Shuang (et al.

)

 

The Functional Assessment of T cells

Pages 177-199

Maruyama, Saho

 

Release of Antibodies and Cytokines from B Cells

Pages 201-207

Liu, Shuang

 

Evaluation of Autoreactive Responses

Pages 209-213

Liu, Shuang

 

Bone Resorption Activity in Mature Osteoclasts

Pages 215-222

Kiyoi, Takeshi

 

Animal Models of Vasculitis

Pages 223-232

Mogi, M.

(et al.

)

 

Design an Intervention Study

Pages 235-241

Hasegawa, Hitoshi

 

Assessment of Disease Activity, Structural Damage, and Function in Rheumatoid Arthritis

Pages 243-250

Hasegawa, Hitoshi

 

Correction to : Production of Lentiviral Particles

Pages E1-E1

Liu, Shuang